stemspan sfem (stem cell tech catalog number 9650) Search Results


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Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
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STEMCELL Technologies Inc stemspan sfem ii human hematopoietic stem cell expansion media
Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
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Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
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STEMCELL Technologies Inc stemspan sfem ii basal medium
Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
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STEMCELL Technologies Inc stemspan sfem ii medium
Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
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Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
Stemspan Sfem Stem Span Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc stemspan™ sfem (stem cell technology)
Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
Stemspan™ Sfem (Stem Cell Technology), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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STEMCELL Technologies Inc stem span sfem culture medium
Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, <t>SFEM:</t> Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: <t>StemSpan</t> CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.
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Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, SFEM: Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: StemSpan CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.

Journal: International Journal of Molecular Sciences

Article Title: Distinct miRNA Signatures and Networks Discern Fetal from Adult Erythroid Differentiation and Primary from Immortalized Erythroid Cells

doi: 10.3390/ijms22073626

Figure Lengend Snippet: Experimental design. A schematic diagram showing cell-type-specific culture stages, conditions, and timelines. hCD34+: primary human peripheral-blood-derived CD34+, HUDEP: cell lines HUDEP-1 and HUDEP-2, SFEM: Stem Span SFEM II, αMEM: Minimum Essential Medium Eagle with alpha modification, IMDM: Iscove’s Modified Dulbecco’s Medium, FBS: fetal bovine serum, SCF: stem cell factor, CC-100: StemSpan CC100, Dexa: dexamethasone, Dox: doxycycline, EPO: erythropoietin, HS: human serum, Holo-Tnf: holo-transferrin, Hep: heparin, 2-ME: 2-mercaptoethanol.

Article Snippet: Cells were again eluted with 5 mL BB and collected by centrifugation at 300× g for 5 min. Isolated cells were then expanded in StemSpan SFEM II (Stem Cell Technologies, Vancouver, Canada) supplemented with 1 μM Dexamethasone (Sigma-Aldrich, Munich, Germany), 2 IU/mL EPO (Binocrit 4000 IU/0.4 mL, Sandoz GmbH, Kundl, Austria), 1% StemSpan CC100 (Stem Cell Technologies, Vancouver, Canada) and 1× Penicillin-Streptomycin (Thermo Fisher Scientific, Waltham, MA, USA) at concentrations below 0.5 × 10 6 cells/mL for ten days.

Techniques: Derivative Assay, Modification